Differentation of closely related Vaccinal Strains of Pasteurella multocida using Polymerase Chain Reaction (PCR)

Abstract

Nucleic acid-based differentiation of closely related Pasteurella multocida vaccinal strains was performed. Morphological and biochemical characterization, HS-specific and species-specific PCR analysis of Pasteurella multocida vaccinal strains were demonstrated useful in distinguishing haemorrhagic septicemia-causing type B strains.  The PCR assay performed for species specific P.  multocida by using primer pair KMT1T7 and KMTISP6 resulted in amplification of all the strains. Another PCR analysis carried out for H.S.  causing strain conformation by using primer pairs KTT72 and KTSP61 showed that only H.S.  causing strains were amplified. It was also observed that PCR amplification performed directly on bacterial colonies or cultures was an extremely rapid, sensitive method of P. multocida identification.