A Comparative Study on Efficiency of Enzyme Linked Immunosorbent Assay and Hemagglutination Inhibition Test in Detecting Serum Antibody Against Avian Influenza Viruses in Commercial Broilers

Authors

  • Sarfraz Ali Fazlani Faculty of Veterinary and Animal Sciences, Lesbela University of Agriculture, Water and Marine Sciences, Uthal, Lesbela, Pakistan
  • Muhammad Abubakar National Veterinary Laboratory, Park Road, Islamabad
  • Faraz Shahid Faculty of Veterinary and Animal Sciences, Lesbela University of Agriculture, Water and Marine Sciences, Uthal, Lesbela, Pakistan
  • Muhammad Irfan National University of Agricultural Sciences, NARC, Park Road, Islamabad, Pakistan
  • Muhammad Javed Arshed National Veterinary Laboratory, Park Road, Islamabad

Keywords:

Avian influenza, Geometric mean titre, Haemagglutination Inhibition Test and ELISA

Abstract

A comparative study was undertaken to assess the diagnostic performance of HI and ELISA tests using a set of serum samples obtained from broilers post Avian Influenza vaccination. The geometric mean titers of HI antibodies as log2 indicated post-vaccination titres after 14 days. ELISA test showed positive results up to 1:16 HI titre, and a linear between these two serological tests was also confirmed. It was concluded that there was a positive relationship between HI and ELISA, although ELISA was more sensitive because it detected low level of antibodies than HI. Also, it was proved that the tests can be used efficiently for the detection of antibodies in the broiler serum samples. However, HAI and titration of antibodies by geometric mean titre was convenient to measure the protective level of antibodies to determine the efficacy of commercially available vaccines. 

Cyclopia in a still born calf

Published

2010-12-31

Issue

Vol. 5 No. 2

Section

Research Articles