Methicilline Resistant Status of Staphylococcus Species Isolated from Mastitic Bovines

Authors

  • Muhammad Imran Faculty of Veterinary Sciences, University of Agriculture, Faisalabad
  • Sajjad Ur Rahman Faculty of Veterinary Sciences, University of Agriculture, Faisalabad
  • Muhammad Abubakar National Veterinary Laboratory, Park Road, Islamabad
  • Murtaz ul Hassan Faculty of Veterinary Sciences, PMAS Arid Agriculture University, Rawalpindi
  • Aaysha Riaz Faculty of Veterinary Sciences, PMAS Arid Agriculture University, Rawalpindi
  • Manzoor Hussain Faculty of Veterinary Sciences, PMAS Arid Agriculture University, Rawalpindi

Keywords:

Staphylococcus, beta-lactamase, methicilline

Abstract

Bacteriological examination of milk samples (n=100) from mastitic buffaloes and cows revealed presence of Staphylococcus aureus in 52 and Staphylococcus epidermidis in 16 samples. Out of these 30.76% of Staphylococcus aureus and 25% of Staphylococcus epidermidis isolates were found positive for beta-lactamase production through nitrocefin test. The isolates were subjected to antimicrobial susceptibility testing through disk diffusion using oxacillin 1µg and cefoxitin 10µg antibiotic disks. Out of total 68 staphylococcal isolates, twenty-two (32.35%) were resistant to these disks hence declared as methicilline-resistant.

Author Biographies

Muhammad Imran, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad

Department of Veterinary Microbiology

Sajjad Ur Rahman, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad

Department of Veterinary Microbiology

Abdominal hernia in a calf

Published

2010-12-03

Issue

Vol. 5 No. 1

Section

Research Articles