Differentiation of Closely Related Isolates (vaccinal strains) of Pasteurella multocida using FT-IR

Abstract

The present study was undertaken to differentiate closely related vaccinal strains (B: 3, 4 and B: 6) of Pasteurella multocida. Observations were made on the biochemistry of whole cell, envelope and OMPs of using analytical technique, FT-IR.  The spectral peaks of whole cells, envelope and OMPs were differentiated between B: 6 and B: 3, 4 by FT-IR analysis. Spectral peaks of B: 3, 4 whole cells were at 644 cm–1 and at absorbance of 5.90, while B: 6 spectral peaks were observed at wave numbers 628, 640 and 651 cm–1 and absorbance at 0.35, 0.66 and 0.67 respectively.  Peaks for B: 3, 4 envelope preparations were at 646 wave number (cm-1) and at absorbance of 0.75 but in B: 6 envelope they were at 628, 640 and 651 wave number (cm-1) and at absorbance of 0.35, 0.43 and 0.24 respectively. The spectral peak was found in B: 3, 4 OMPs at 646 wave number (cm-1) and at absorbance of 5.90, while spectral peaks for B: 6 OMPs were at 628, 640 and 651 wave number (cm-1) and at absorbance of 0.60, 0.50 and 0.21 respectively.  It was concluded that the technique applied in this study was useful for better understanding of the structural configurations of the organism. The data generated will help in development of good vaccines which will help in control of HS disease in cattle, detailed studies with a large number of isolates is warranted.