Antibiogram Profile and 16S rDNA RFLP Analysis of Enteric Bacteria from Farm Animals of Andaman Islands
Keywords:
Antibiotic sensitivity, bacterial isolates, farm animals and genetic characterization, faecal swabAbstract
A total of 63 bacterial isolates were isolated from faecal swab of cattle, goat and poultry from different areas of Andaman. The majority of the isolates were E. coli (37.93%), however, other bacteria viz. Salmonella, Enterobacter, Klebsiella, Pantoea, Serratia, Yersinia spp. were also isolated. The antibiotic sensitivity profiling revealed great variation in the sensitivity pattern. The RFLP pattern of the microorganisms revealed different restriction profile with the restriction enzyme. The result indicated that bacterial isolates from the gut faecal swab were genetically diverse. PCR-RFLP could be used as a rapid and accurate tool for the diversity analysis of bacterial isolates from faecal swab.