SNP Genotyping of DGAT1 Gene in Bubalus bubalis

Abstract

Present study was conducted to validate six out of  nineteen earlier reported [20] Single Nucleotide  Polymorphisms (SNPs) in acyl-CoA:  diacylglycerol acyltransferase1 (DGAT1) - intron  I gene fragment in Mehsana buffalo, to explore  some novel SNPs in the selected gene fragment of  this species and finally analyse the correlation of  the considered and explored SNP’s with milk fat  percentage and milk yield. Animals were ranked according to their production in terms of milk yield and milk fat percentage and clubbed into two tail classes. DGAT1- intron I of Mehsana buffalo was amplified from nucleotide position 1511 to 2657, giving an amplicon size of 1146bp, to cover six out of nineteen earlier reported SNPs at nucleotide positions viz. 1606, 1784, 1875, 2141, 2217 and 2394. All the SNPs except 2217 were found to be monomorphic and SNP 1606 exhibited allele C instead of wild allele T as reported in an earlier study (Mishra et. al. 2007).  Four novel SNPs at nucleotide positions 2099, 2214, 2219 and 2370 were detected. All the SNPs were correlated with MFP and MY, however, no significant association of any SNP considered and explored in the present study was found with any of the two milk production traits. The study suggests that the SNPs analysed do not have any influence on milk yield and milk fat percentage and might be used as genetic markers for Mehsana buffaloes.