Anti-Diarrhoeal, Antimicrobial and Cytotoxic Effect of Ethanol Extracted Guava (Psidium guajava) Leaves

Authors

  • Mohammad Mahmudul Hassan Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh
  • A B M Shahinuzzaman Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh
  • Shahneaz Ali Khan Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh
  • M B Uddin Sylhet Agricultural University, Sylhet-3100, Bangladesh
  • Mohammad Mahabub-Uz-Zaman BCSIR Laboratory, Chittagong-4220, Bangladesh

Keywords:

Anti-diarrhoeal, antimicrobial, cytotoxicity, ethanol extracted guava leaf, albino rat

Abstract

Guava leaf (Psidium guajava) is commonly used to treat bacterial infection, gastroenteritis and diarrhoea. Present study was aimed at determining the anti-diarrhoeal, antimicrobial and cytotoxic effect of the ethanol extracted guava plant leaves. The ethanol extracted guava (P. guajava) plant leaves showed significant (P<0.05) anti-diarrhoeal activity on castor oil induced diarrhoea in model albino rat comparing with loperamide (P<0.01). The leave extract tested against ten pathogenic bacteria and exhibits prominent antibacterial activity against five-gram positive (Staphylococcus aureus, Bacillus polymyxa, Bacillus cereus, Bacillus megnatum and Bacillus subtilis) bacteria and one gram negative (10.5mm). bacteria with highest zone of inhibition(13.25mm) for Proteus sp followed by Staphylococcus aureus (10.5mm). LC50 of ethanol extract of guava leaves against brine shrimp nauplii was 828.7μg/ml and the wide range of LC50 value denoted the safety effect of the extract.

Author Biographies

Mohammad Mahmudul Hassan, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh

Department of Physiology, Biochemistry and Pharmacology

A B M Shahinuzzaman, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh

Department of Physiology, Biochemistry and Pharmacology

Shahneaz Ali Khan, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong-4202, Bangladesh

Department of Physiology, Biochemistry and Pharmacology

M B Uddin, Sylhet Agricultural University, Sylhet-3100, Bangladesh

Department of Medicine and Surgery

Mohammad Mahabub-Uz-Zaman, BCSIR Laboratory, Chittagong-4220, Bangladesh

Scientific Officer, Drug and Toxins Research Division

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Published

2011-12-31

Issue

Vol. 6 No. 2

Section

Research Articles

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